Stem Cells are basic cells present in abundance in our body and capable of differentiating into all types of cells.
These cells have shown promising results when used for treatment of several diseases for which cure is not known.
Stem cell sources are:
- Umbilical cord blood (UCBS)
- Umbilical cord tissue derived stem cells or Whartonís jelly Mesenchymal Stem Cells (WJMSCs)
- Placental Tissue derived stem cells (PSCs)
- Autologous bone marrow (BMSC)
- Menstrual Blood derived stem cells (MBSC)
- Urine derived Stem cells (USCs)
- Dental stem cells (DSCs)
- Tissue specific stem cells (TSSCs)
Our medical department evaluates each patient who is interested in stem cell therapy. Reviewing the patientís full medical information, our medical department doctors and scientist recommend source and type of stem cells should be used for treatment.
Umbilical Cord Blood Stem Cells (UCBS)
Therapy for cerebral palsy, muscular atrophy, ataxia, neurodegenerative diseases and optic nerve conditions, we transplant UCBS (Autologous or HLA match. Our UCBS enriched injection consists of three subsets of stem cells.
I) Hematopoietic stem cells (CD34+),
II) Endothelial progenitor cells (CD31or Tie 2), and
III) Mesenchymal stem cells (CD90++ CD271+).
Animal and clinical studies shown that hematopoietic stem cells likely to form tissue specific cells in the body. Endothelial progenitor stem cells induce neovascularization in tissue. Mesenchymal stem cells can assist in the growth of tissue specific cells e.g. liver cells, kidney cells, chondrocytes, and neurons, and also repairs in relation to vascular disorders within the brain, ocular areas, and throughout the body including the heart, kidney, and pancreas. However, It is believed that stem cells secrete several growth factors, chemokines, metaloprotenases that trigger regenerative response in tissue.
Umbilical cord tissue derived stem cells or Whartonís jelly Mesenchymal Stem Cells (WJMSC)
Recent research publications and in house experience, we utilize allogeneic WJMSC for Osteoartheristis, and autoimmune disorders. In these therapies GMP manufactured WJMSC are used for transplantation. Mesenchymal stem cells not only produce important growth factors and differentiate into desired cell types but can also regulate the immune system (Immunomodulation), reducing inflammation, scarring, and cell apoptosis.
Placental stem cells (PSC)
Multipotent cells isolated from human placenta (PSC) have been known to differentiate into Epithelial, endothelial and mesodermal lineage. PSC have been shown to treat diseases as, Parkinsonís and Alzheimerís disease, autoimmune disorders, stroke, lupus, muscular dystrophy and Liver diseases. They have relatively weak antigens on their surfaces so they can be used in a wide spectrum of potential recipients. Placental stem cells have powerful "homing" ability, which enable them to home in areas of the body that are in need of repair.
Bone Marrow Stem Cells (BMSC)
Disorders like Spinal Cord Injuries, Cerebral Palsy, Autism, Traumatic Brain Injury, Diabetic Foot, Femur Head Necrosis, and Heart Diseases, We may offer use of the patientís own bone marrow stem cells (depending on the patientís condition). In GMP facilityBMSC can be enriched from bone marrow and create three to five separate injections depending upon disease state. These injections have Epithelial, endothelial and mesenchymal stem cells. Use of autologous Bone marrow is viable option for those who would only like to utilize their own stem cells without any side effects.
Menstrual Blood derived stem cells (MBSC)
Menstrual blood has been discarded as unsanitary waste. Every month during a womanís menstrual period the Endometrium (uterine lining) is shed along with the extra blood and tissues. This lining of the uterus contains a large number of stem cells with characteristics similar to bone marrow. Recent research demonstratesthat the menstrual blood contains large number of self-renewing stem cells, which can multiply rapidly, and able todifferentiate into many other types of cells such as cardiac, neuronal, bone, fat, cartilage cells thus exhibit potential source for stem cell therapy.
MBSCare capable of differentiating into other cell type in the human body when transplanted. Menstrual stem cell technologyhas not yet been utilized to date in human therapies but ongoing research shown its potencial use in stem cell therapy. It is possible that in the futureseveral diseases like Alzheimer's Disease, Atherosclerosis, Diabetes, Heart Disease,Inflammatory Bowel Disease, Parkinson's Disease, Osteoarthritis, Rheumatoid Arthritis and many others can be treated using MBSC.
Menstrual bloodstem cells are can be easily collected, processed and harvested in an affordable, painless and non-invasivemanner.
Urine derived Stem cells (USCs)
This is a novel cell source of stem cell, andisolation process requires 24hrs urine collected in sterile tubes, which will be transported in the lab where cells will be isolated by centrifugation.USCs provide advantages for cell therapy and tissue engineering applications in regeneration of various tissues, particularly in the genitourinary tract, because they originate from the urinary tract system. Preclinical outcomes of cell therapy with USCs have been positive in models of diabetic erectile function, stress urinary incontinence, urethra and bladder reconstruction, and renal insufficiency.Importantly, USCs can be obtained via a non-invasive, simple, and low-cost approach and induced with high efficiency to differentiate into three dermal cell lineages.Besides genitourinary tissue repair, USCs might also be a viable cell source for cell-based therapy in treatment of tissue defects or diseases in other systems.
Dental stem cells (DSCs)
DSCs derived from human dental tissues were well established over a decade ago.
Four types of human dental stem cells have been isolated and characterized:
I) Dental pulp stem cells (DPSCs)
II) Stem cells from exfoliated deciduous teeth (SHED)
III) Stem cells from apical papilla (SCAP)
IV) Periodontal ligament stem cells (PDLSCs)
These dental stem cells are considered as mesenchymal stem cells (MSCs) and possess different levels of capacities to become specific tissue forming cells.
DSCs are capable of differentiating into odontoblast-like cells,osteoblasts, adipocytes, smooth and skeletal muscle cells. For example, DSCs may form a woven bone complexin culture appropriate conditions, which can be remodeled into lamellar bone after transplantation in animal models. DSCs express (nestin and GFAP),which are molecules related to the neural crest-cell origin of thedental pulp.
The multipotent stem cell content of the DSCs indicatesthat this tissue has a big potential for clinical applications. It offersthe opportunity to isolate stem cells non invasively (Without surgical procedure).DSCs are multipotent mesenchymal type of stem cells that have the future potential to differentiate into a variety of other cell types including cardiomyocytes to repair damaged cardiac tissue following a heart attack, neurons to generate nerve and brain tissue, myocytes to repair muscle, osteocytes to generate bone, chondrocytes to generate cartilage and adipocytes to generate fat.
Tissue specific stem cells (TSSC)
Many adult tissues contain stem cells that can replace cells that die or restore tissue after injury. Skin, muscle, intestine and bone marrow, for example, each contain their own stem cells. Extensive animal and clinical studies are currently ongoing for treatment of diseases such as arthritis, non-healing bone fractures, skin burn and liver diseases. It is also possible that these cells modulate the immune system in response to injury. Under GMP protocol, tissue specific TSSC can be generated from patient tissue. It is also possible that these cells aid in the regeneration of tissues.
Stem cells Treatment Methods
Intravenous Injection (IV)
This is a very simple process and familiar to most patients. A tubing line with a catheter tip threaded over a needle is placed into the patientís vein. Once proper placement is obtained, the needle portion is removed and the flexible plastic catheter is left in place in the vein with the tubing attached. The stem cell solution will be administered through the IV. Typically no sedation is required for this procedure. The entire IV injection process takes less than 30 minutes to complete.
Lumbar Puncture or Intrathecal Injection
In this procedure stem cell transplantation is done using hollow needle, which is inserted between two of the spinal bones (L4-L5) and into the spinal canal. This is a procedure to access the cerebrospinal fluid of the brain and spinal cord and helps to deliver stem cells directly into the cerebral spinal fluid, bypassing the blood-brain barrier. Our medical team has determined that this is the least invasive method for delivering stem cells directly into the central nervous system. Cerebrospinal Fluid is used to provide protection for the brain and spinal cord, limiting the possibility of injury to these areas. The body constantly produces CSF and thus any withdrawn fluid is naturally replaced within a few hours.
Local injections can be used to transplant tem cells in variety of conditions. These include spinal cord injuries, peripheral neuropathy, diabetic foot, autoimmune disorders and muscular dystrophy. For local injections, BMSC /WJMSC/PSC/ TSSC are transplanted by multiple injections directly into and around the injury site.
Intravascular Interventional Injection
Intravascular interventional injection can be applied to treat some cerebrovascular, cardiovascular diseases, peripheral vascular diseases, Liver diseases, kidney diseases and pancreatic diseases. Stem cells will be directly transplanted into the damaged areas.
The Effects of Stem Cell Treatment
1. Rapid and substantial improvement of mental and physical activity, stabilization and improvement of mood, positive Psychophysiological changes, particularly related to attention, memory, and thinking. Over all improvement in quality of life.
2. Restoration of impaired functions, such as vision, movement, coordination and balance.
3. Restoration of hematopoiesis.
4. Immune competence restoration: Recovery of immune competence in cases of suppressed immunity. Temporary suppression of immune activity in acute autoimmunity
5. Stimulation of tissues and organs trophism, tissue quality improvement, anti-aging and functional rejuvenation.
6. Normalization of metabolic homeostasis, i.e. lipid, carbohydrate, protein, mineral and water metabolism, and hormonal balance.